PLCγ2 regulates osteoclastogenesis via its interaction with ITAM proteins and GAB2
J. Clin. Invest. Dailing Mao, et al. 116:2869 doi:10.1172/JCI28775 [Go to this article.]

Figure 4
PLCγ2 regulates NFATc1 levels and the expression of early osteoclastogenic genes. (A–D) Real-time PCR analysis of osteoclastogenic markers in WT and Plcg2^–/– cells in culture with M-CSF alone (d0) or with RANKL (100 ng/ml) plus M-CSF (10 ng/ml) for 4 days (d4). Data are normalized relative to β-actin. ND, not detected. Significant differences compared with WT are shown. (E) Expression levels of NFATc1 protein in total cell lysates from day 4 WT and Plcg2^–/– OCs, starved for 6 hours and stimulated with RANKL for 0, 5, 20, and 60 minutes. PLCγ1 and PLCγ2 levels are shown. β-Actin served as loading control.