PLCγ2 regulates osteoclastogenesis via its interaction with ITAM proteins and GAB2
J. Clin. Invest. Dailing Mao, et al. 116:2869 doi:10.1172/JCI28775 [
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Figure 3PLCγ2 is required for osteoclastogenesis. (
A) Bone nodule formation in WT and
Plcg2–/– OBs cultured with ascorbic acid and β-glycerolphosphate. (
B) Double labeling of calvarial bones showing the degree of bone formation (BFR) of WT and
Plcg2–/– mice injected on day 0 and day 7 with calcein (WT BFR, 0.8938 ± 0.1042 μm
3/μm
2/d; WT mineral apposition rate [MAR], 0.5927 ± 0.0219 μm/d;
Plcg2–/– BFR, 0.8450 ± 0.1629 μm
3/μm
2/d;
Plcg2–/– MAR, 0.4015 ± 0.0876 μm/d). (
C) WT and
Plcg2–/– BMMs cultured with WT and
Plcg2–/– OBs in the presence of 10
–8 M 1,25 Vit D
3. After 14 days, cells were fixed and TRAP stained to detect the presence of multinucleated OCs. (
D) TRAP-stained OCs generated with RANKL (100 ng/ml) and M-CSF (10 ng/ml) for 5 days (top panel) and bone resorptive pits generated from OCs plated on dentin for 7 days. Pits were stained with hematoxylin red. (
E) WT and
Plcg2–/– BMMs retrovirally transduced with vector alone (pMX) or with Flag-tagged PLCγ2 were allowed to differentiate in osteoclastogenic media for 5 days, then stained for TRAP. Magnification, ×200 (
A,
C–
E); ×100 (
B).
