Gain-of-function mutant of angiotensin II receptor, type 1A, causes hypertension and cardiovascular fibrosis in mice
J. Clin. Invest. Sandrine Billet, et al. 117:1914 doi:10.1172/JCI28764 [Go to this article.]

Figure 8
Cardiovascular characterization of AT_1AMUT and AT_1AWT mice. (A) Vascular morphology was assessed by morphometric analysis of the wall thickening ratio (S/L) in renal arteries in 5-month-old AT_1AWT (white bar) and AT_1AMUT (black bar) littermates. Three mice were studied in each group, and a mean of 20 arteries were measured in each mouse. *P < 0.05 compared with AT_1AWT. (B) Cardiac hypertrophy was assessed by the ratio of heart weight to body weight (HW/BW) in 6- to 10-month-old AT_1AWT (white triangles) and AT_1AMUT (black circles) littermates. (C) Quantitative RT-PCR of ANF and BNP mRNA in AT_1AWT (white bars) and AT_1AMUT (black bars) hearts. (D) Myocardial sections from 5-month-old AT_1AWT and AT_1AMUT mice were stained with Sirius red. Scale bar: 10 μm. (E) Quantitative RT-PCR of collagen αI and PAI1 mRNA in AT_1AWT (white bars) and AT_1AMUT (black bars) hearts. **P < 0.01 compared with AT_1AWT.