Impaired regulation of NF-κB and increased susceptibility to colitis-associated tumorigenesis in CYLD-deficient mice
J. Clin. Invest. Jun Zhang, et al. 116:3042 doi:10.1172/JCI28746 [Go to this article.]

Figure 4
Comparison of TRAF2 and RIP polyubiquitination levels in Cyld^–/– and wild-type cells. (A) Peritoneal macrophages stimulated with TNF-α. TRAF2 ubiquitination was analyzed by immunoprecipitation of the proteins from denatured cellular lysates followed by immunoblotting with an anti-ubiquitin Ab. (B) Lysates prepared from peritoneal macrophages stimulated with TNF-α were immunoprecipitated with an RIP-specific Ab followed by immunoblotting with an anti-ubiquitin Ab.