Mutations within Sox2/SOX2 are associated with abnormalities in the hypothalamo-pituitary-gonadal axis in mice and humans
J. Clin. Invest. Daniel Kelberman, et al. 116:2442 doi:10.1172/JCI28658 [Go to this article.]

Figure 1
Pituitary hormone levels and immunohistochemistry in Sox2 heterozygous mice. (A) GH and LH RIAs on pituitary protein extracts from 6 wild-type (XY^+/+) and 5 Sox2 heterozygous (XY^+/–) 2-month-old littermates (GH, P = 0.004; LH, P = 0.002) and 10 wild-type and 9 Sox2 heterozygous 18.5 dpc littermates (P = 0.002). Both GH and LH were affected; GH deficiency appeared before birth. (B–E) Immunohistochemistry for GH (B and D) and LH (C and E) on pituitary sections from 3-month-old (B and C) and 18.5 dpc (D and E) wild-type and Sox2 mutant littermates. Ant, anterior lobe; Int, intermediate lobe; Post, posterior lobe. Note the presence of extra clefts in the adult sections (arrows in B and C). Staining was clearly reduced in heterozygotes at 18.5 dpc (D and E). Scale bar: 0.3 mm (B and C); 0.05 mm (D and E).