Chronic activation of the prostaglandin receptor EP4 promotes hyaluronan-mediated neointimal formation in the ductus arteriosus
J. Clin. Invest. Utako Yokoyama, et al. 116:3026 doi:10.1172/JCI28639 [
Go to this article.]
Figure 4EP4/PKA stimuli profoundly increased the level of Has2 transcripts in the DA. (
A) Time-dependent HAS isoform mRNA induction by ONO-AE1-329 (10
–6 M).
n = 4–8; *
P < 0.05, **
P < 0.01,
#P < 0.001 compared with control. (
B) Fold increase in HAS2 mRNA expression after 24-hour stimulation with ONO-AE1-329 (10
–6 M) in the absence or presence of H89 (10
–5 M), pCPT-cAMP (10
–4 M), TGF-β (10 ng/ml), and PDGF-BB (10 ng/ml).
n = 4–8. **
P < 0.01,
#P < 0.001 compared with control.
†P < 0.05,
††P < 0.01 compared with ONO-AE1-329.
