Fatty acid amide hydrolase deficiency limits early pregnancy events
J. Clin. Invest. Haibin Wang, et al. 116:2122 doi:10.1172/JCI28621 [Go to this article.]

Figure 2
Expression of Nape-pld and Faah in the oviduct and preimplantation embryos. (A) Spatiotemporal expression profiles of Nape-pld and Faah mRNA in the oviduct on days 1–4 (D1–4) of pregnancy. The results show that Nape-pld and Faah are expressed in a cell- and region-specific manner in the mouse oviduct during the preimplantation period. Higher levels of Nape-pld were detected in the oviduct epithelium at the isthmus region but much lower levels in the ampullary region. In contrast, Faah was primarily expressed in the epithelium at the ampullary region, with lower expression in the isthmus. Scale bar: 25 μm. (B and C) Cell-specific expression of Nape-pld and Faah mRNA and protein expression in preimplantation embryos. The results show that NAPE-PLD and FAAH are differentially expressed in the preimplantation embryos. While NAPE-PLD was expressed at all stages of preimplantation embryos, from fertilized 1-cell embryos to blastocysts, FAAH was expressed upon activation of the embryonic genome at the 2-cell stage and beyond. NAPE-PLD protein was detected in both nuclei and cytoplasm by this commercially available antibody. FAAH protein was detected in the cytoplasm and was primarily localized to outside cells in morulae and blastocysts. Images depict TRITC-labeled antigens as red, SYTO-13–labeled nuclei as green, and merge as yellow (scale bar: 50 μm). RT-cont, RNA without RT reaction; Pr-cont, primer control.