Fatty acid amide hydrolase deficiency limits early pregnancy events
J. Clin. Invest. Haibin Wang, et al. 116:2122 doi:10.1172/JCI28621 [
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Figure 1Impact of marijuana exposure on oviductal transport, early embryo development, and implantation in wild-type mice. (
A) Number of mice with oviductal embryo retention per total number of mice examined on day 4 of pregnancy. (
B) Percentage of embryos recovered from oviducts or uteri. (
C) Differential distribution of morulae and blastocysts among drug-treated groups. Statistical significance between treatment groups was evaluated using unpaired 1-tailed Student’s
t test (*
P < 0.01). (
D) TUNEL analysis in blastocysts upon maternal exposure to THC in vivo. Images shown depict FITC-labeled apoptotic cells as green, propidium iodide–labeled nuclei as red, and merge as yellow (scale bar: 20 μm). (
E and
F) Implantation in mice receiving different drug treatments. Implantation sites (IS) were visualized by the blue-dye method on day 5 mid-morning. Numbers within the bars in
E indicate the number of mice with implantation sites per total number of mice examined. The results show that while THC, the most psychoactive component of marijuana, but not the inactive CBD and CBN, derails oviductal embryo transport, retards preimplantation embryo development, induces apoptosis in blastocysts, and blocks implantation in wild-type mice, SR141716 (SR1, a selective CB1 antagonist) largely reduces the detrimental effects of THC exposure. Veh, vehicle.
