Selective tyrosine kinase inhibition by imatinib mesylate for the treatment of autoimmune arthritis
J. Clin. Invest. Ricardo T. Paniagua, et al. 116:2633 doi:10.1172/JCI28546 [Go to this article.]

Figure 4
Inhibition of macrophage c-Fms and downstream MAPK pathways by imatinib. (A and B) Isolated resident peritoneal macrophages were serum starved, preincubated with imatinib, and stimulated with 100 ng/ml M-CSF for 10 minutes in the presence of imatinib and lysates generated for IB analysis. IBs were probed with antibodies specific for phospho–c-Fms and total Fms (A) or phospho–Akt (Ser473) and total Akt (B). (C) Peritoneal macrophage lysates generated using the stimulation conditions described in A and B were printed on RPP arrays. RPP arrays were probed with a variety of antibodies specific for MAPK pathway and other protein tyrosine kinases, and normalized kinase levels displayed as a heatmap.