Nonsense-mediated mRNA decay affects nonsense transcript levels and governs response of cystic fibrosis patients to gentamicin
J. Clin. Invest. Liat Linde, et al. 117:683 doi:10.1172/JCI28523 [
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Figure 3Effect of NMD inhibition on the transcript level of physiologic NMD substrates in nasal epithelial cell lines. The level of each analyzed transcript was measured by real-time PCR and normalized to that of
GAPDH and the ratio between these normalized levels following treatment was calculated and compared to the ratio in untreated cells. (
A–
E) Effect of indirect NMD inhibition by CHX on the level of
RPL3 (
A),
SC35 1.7 kb (
B),
SC35 1.6 kb (
C),
ASNS (
D), and
CARS (
E) transcripts. (
F–
J) Effect of direct NMD inhibition by
UPF1 downregulation on the level of
RPL3 (
F),
SC35 1.7 kb (
G),
SC35 1.6 kb (
H),
ASNS (
I), and
CARS (
J) transcripts. The increase in the level of
CFTR transcripts is shown as mean ± SEM.
