Rosiglitazone promotes development of a novel adipocyte population from bone marrow–derived circulating progenitor cells
J. Clin. Invest. Joseph T. Crossno, et al. 116:3220 doi:10.1172/JCI28510 [Go to this article.]

Figure 7
GFP⁺ adipocytes do not express CD45 or CD11b. PBMCs and omental adipose tissue were isolated from GFP⁺ BMT mice fed ROSI-impregnated chow for 7 weeks. The adipose tissue was digested with collagenase and nonbuoyant stromal/vascular cells, and buoyant adipocytes were separated by differential centrifugation. All 3 cell fractions were stained with APC-conjugated anti-CD45.2 (clone 104-2) antibodies and PE-conjugated anti-CD11b (clone M1/70) antibodies. The stained cell suspensions were subjected to FACS analysis for GFP⁺ cells (GFP^– cells were excluded) expressing CD45 and/or CD11b. Representative scattergrams are shown, and the average percentage (from 3 independent sorts) of cells is indicated in the top left (CD11b⁺), top right (CD45⁺ and CD11b⁺), and bottom right (CD45⁺) quadrants. Gates were set and data were corrected using unstained cell suspensions or suspensions incubated with APC- and PE-conjugated isotype-matched control antibodies.