Rosiglitazone promotes development of a novel adipocyte population from bone marrow–derived circulating progenitor cells
J. Clin. Invest. Joseph T. Crossno, et al. 116:3220 doi:10.1172/JCI28510 [Go to this article.]

Figure 6
GFP⁺ ML adipocytes express C/EBPα, PPARγ, adiponectin, FABP, perilipin, leptin, and β3-AR but not UCP-1 and have high mitochondrial content. (A) cDNA was prepared from RNA from white (from omental adipose tissue), brown (from dorsal intrascapular adipose tissue), and ML adipocytes isolated by collagenase digestion and flotation as described in Methods. Equal amounts of cDNA (1 μg) were subjected to PCR with validated primer sets for the targets indicated to the left of each gel photograph. PCR reactions were then resolved on 2% agarose gels run in the presence of ethidium bromide. Fluorescence photographs of the gels were captured to computer, and band intensities were measured using ImageJ software. Representative gel photographs are shown in the left column. Densitomentry data were averaged over 3 experiments and corrected for differences in β-actin levels. Average band intensities are shown in the corresponding bar graphs to the right of each gel photograph. (B) Mitotracker Red 580 staining was performed on minced white adipose tissue fragments from GFP⁺ BMT mice fed ROSI-impregnated chow for 7 weeks. Representative fluorescence deconvolution images for GFP and Mitotracker signals, as well as a digital overlay of GFP and Mitotracker signals, are shown (yellow: GFP plus Mitotracker; red or orange: Mitotracker plus little or no GFP). The general location of white (W) and ML adipocytes is indicated by the white ovals.