Rosiglitazone promotes development of a novel adipocyte population from bone marrow–derived circulating progenitor cells
J. Clin. Invest. Joseph T. Crossno, et al. 116:3220 doi:10.1172/JCI28510 [Go to this article.]

Figure 5
Microscopic observation of GFP⁺ ML adipocytes isolated by collagenase digestion. (A) Omental and dorsal intrascapular adipose tissue was isolated from GFP⁺ BMT mice fed ROSI-impregnated chow for 7 weeks. The tissue was digested with collagenase, and adipocytes were isolated by flotation. Adipocytes were then subjected to flow sorting to separate GFP⁺ and GFP^– cells. Isolated cells were examined by phase-contrast and fluorescence digital deconvolution microscopy to evaluate morphology and GFP expression. Shown are representative phase-contrast and fluorescence images of GFP⁺ ML adipocytes (MLAs) compared with a GFP^– unilocular white adipocyte (from omental tissue) and a GFP^– ML brown adipocyte (from dorsal intrascapular brown fat). Digital overlay of GFP fluorescence signal and phase-contrast images in shown. Scale bar (red): 100 μm. (B) Phase-contrast, fluorescence, and digital overlay images of adipocytes isolated by collagenase digestion and flotation from GFP⁺ BMT mice fed ROSI-impregnated diet for 7 weeks. The image shows the substantial number of GFP⁺ adipocytes present in the total adipocyte population.