Rosiglitazone promotes development of a novel adipocyte population from bone marrow–derived circulating progenitor cells
J. Clin. Invest. Joseph T. Crossno, et al. 116:3220 doi:10.1172/JCI28510 [Go to this article.]

Figure 3
FACS analysis of GFP⁺ adipocytes. Adipocytes were isolated from a nontransgenic, nontransplanted (WT C57BL/6) mouse (as a negative control); a UBI-GFP/BL6 transgenic (UBI-GFP Tg) donor mouse (as a positive control); and untreated GFP⁺ BMT mice (Cntrl), ROSI-treated mice, or mice fed a high-fat diet for 7 weeks. Adipocytes were isolated by collagenase digestion and flotation from omental or dorsal intrascapular depots. Shown are representative scattergrams in which green dots indicate GFP⁺ cells and black dots represent either non-GFP⁺ cells, free lipid droplets, or debris. The average percentage of particles that were GFP⁺ is indicated at the top right of each scattergram. The results demonstrate that ROSI increases the number of GFP⁺ adipocytes in the tissue samples. High-fat diet also increases GFP⁺ adipocyte numbers but to lesser extent than ROSI treatment. GFP comp, GFP compensation.