Rosiglitazone promotes development of a novel adipocyte population from bone marrow–derived circulating progenitor cells
J. Clin. Invest. Joseph T. Crossno, et al. 116:3220 doi:10.1172/JCI28510 [Go to this article.]

Figure 2
Appearance and distribution of GFP⁺ ML adipocytes in adipose tissue from untreated, ROSI-treated, and high-fat diet–fed mice. (A) Five-micrometer sections were prepared from paraffin-embedded omental (left 3 columns) and dorsal intrascapular (right column) adipose tissue from GFP⁺ BMT animals fed control, ROSI-impregnated, or high-fat diets for 3 weeks. Sections were deparaffinized, rehydrated, and mounted with aqueous mounting medium. Sections were examined by phase-contrast and fluorescence digital deconvolution microscopy. The GFP fluorescence signal was digitally overlayed on the corresponding phase-contrast image. Representative photomicrographs of both white fat (left 3 columns) and brown fat (Brn fat) are shown. Scale bar (red): 100 μm. (B) Serial sections of omental white fat from GFP⁺ BMT mice fed ROSI for 3 weeks were compared for GFP fluorescence and immunohistochemical staining for GFP (GFP Ab). Lack of staining with an isotype-matched negative control antibody (Iso match Ab) is also shown.