Injury-induced innate immune response in human skin mediated by transactivation of the epidermal growth factor receptor
J. Clin. Invest. Ole E. Sørensen, et al. 116:1878 doi:10.1172/JCI28422 [
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Figure 5Fold induction of hBD-3 mRNA in ex vivo–wounded skin determined by qRT-PCR. The relative expression of hBD-3 mRNA was analyzed by real-time qRT-PCR on skin samples from 5 different donors. The expression of hBD-3 was normalized to the housekeeping gene G3PD. The treatment with AG-1478 did not influence the G3DP levels. The expression of hBD-3 at day 0 was set to 1. Horizontal lines denote the average induction of the 5 donors. The donor with a 27-fold increase even after treatment with AG-1478 was the same donor who had a 631-fold induction of hBD-3. Thus, even in this donor, AG-1478 dramatically inhibited hBD-3 expression. The donors included in this figure were all responders.
