Liver X receptors regulate adrenal cholesterol balance
J. Clin. Invest. Carolyn L. Cummins, et al. 116:1902 doi:10.1172/JCI28400 [Go to this article.]

Figure 5
LXR effects in mouse and human adrenal cells. (A) Northern blot analysis of ABCA1 and StAR from mouse Y1 cells treated with vehicle, LXR agonist T1317 (1 μM), or RXR agonist LG268 (1 μM) for 6 hours. Quantitation was performed by densitometry using the largest transcript for StAR normalized to β-actin. (B) Western blot analysis of StAR from Y1 cells treated with or without 1 μM LXR agonist T1317 (20 μg per lane, n = 4) for 24 hours. (C) QPCR analysis from human H295R cells treated with vehicle, T1317 (1 μM), LG268 (1 μM), or both ligands for 24 hours. Data represent the mean ± SD (n = 3). Cycle times for the highest expressing group for each gene are shown in the corresponding bar. *P < 0.05, significantly different from control.