The A_2B adenosine receptor protects against inflammation and excessive vascular adhesion
J. Clin. Invest. Dan Yang, et al. 116:1913 doi:10.1172/JCI27933 [Go to this article.]

Figure 4
E-selectin, P-selectin, and ICAM-1 levels are augmented and IκB-α levels are reduced in the A_2B AR-KO mice. (A) Western blot analysis of protein extracts isolated from mesenteric arteries derived from WT and KO mice. Despite attempts to dissect an endothelial layer from a mouse artery, the preparation was accompanied with VSMCs. vWF is only expressed in endothelial cells and, hence, serves as an appropriate marker for this cell type. Samples were subjected to Western blotting with the indicated antibodies. (B) Quantification of the Western blot analyses of WT and A_2BAR-KO samples shown in A. Probing with anti-actin was used to generally reflect protein loading. Protein levels on the Western blots were assessed and normalized to the level of vWF, which represents the fraction of endothelial cells within the loaded protein. Quantification was performed using NIH ImageJ software (version 1.62; http://rsb.info.nih.gov/ij/). The data shown represents the average ± SD of 3 experiments.