Ectopic pancreas formation in Hes1 -knockout mice reveals plasticity of endodermal progenitors of the gut, bile duct, and pancreas
J. Clin. Invest. Akihisa Fukuda, et al. 116:1484 doi:10.1172/JCI27704 [Go to this article.]

Figure 6
The fate of ectopically activated Ptf1a -null cells in the prospective CBD, MDP, and distal stomach inHes1^–/–Ptf1a^cre/creRosa26r double-null mice. (A–H) The CBD and MDP region in Hes1^–/–Ptf1a^cre/creRosa26r double-null mice at E17.5. Macroscopic view of the CBD region stained with X-gal (arrow in A). Double immunostaining showed that X-gal–positive cells at the MDP region were positive for insulin and glucagon (arrows in B and C). (C) α-SMA immunostaining showed ectopic pancreatic endocrine cells penetrating the duodenal wall at the MDP region. Cdx2 immunostaining showed that Cdx2/X-gal double-positive cells (arrows in inset, D) were present in the duodenal epithelium. (E–H) Histological analysis of the CBD region in Hes1^–/–Ptf1a^cre/creRosa26r double-null mice on the thin sections. Arrows indicate coexpression of β-galactosidase and pancreatic markers. (I–K) The distal stomach in Hes1^–/–Ptf1a^cre/creRosa26r double-null mice at E17.5. Many of the X-gal–positive cells in the stomach were PAS positive (arrows in I) and weakly cytokeratin positive (dashed lines in I), indicating differentiation into stomach epithelial cells. A subset of X-gal–positive cells expressed gastrin (J) and insulin (K). Scale bars: 50 μm. Original magnification, ×40 (A).