Ectopic pancreas formation in Hes1 -knockout mice reveals plasticity of endodermal progenitors of the gut, bile duct, and pancreas
J. Clin. Invest. Akihisa Fukuda, et al. 116:1484 doi:10.1172/JCI27704 [Go to this article.]

Figure 3
Ectopic pancreas formation in the CBD and MDP in Hes1^–/– Ptf1a^cre/wt ROSA26r mice. Macroscopic and microscopic views of the X-gal–stained CBD at E17.5 show that the Ptf1a lineage–negative normal CBD in Hes1^WT/WT mice (arrows in A) was replaced by Ptf1a lineage–labeled pancreatic tissue in Hes1-null mice (arrows in B and D). Note that the duodenum is light blue because of endogenous β-galactosidase activity. The converted pancreatic tissue extended from the site where the cystic duct would have joined the CBD to the junction with the duodenum (B). Note that the native ventral pancreas (arrow in C) and rare cells just beside the CBD (arrowhead in C) were Ptf1a lineage labeled in Hes1^WT/WT mice. The DBA-positive CBD epithelium at the MDP (E) was replaced by DBA-negative, Ptf1a lineage–labeled pancreatic tissue in Hes1-null mice (F). Neither amylase- nor insulin-producing cells were present in the CBD in Hes1^WT/WT mice (G). (H) Double immunostaining showed that the ectopic pancreatic tissue at the MDP was positive for amylase and insulin in Hes1-null mice (note that F and H are adjacent sections). The normal structure of the MDP is shown (I). (J) Ectopic pancreatic tissue penetrated the duodenal wall at the MDP, shown by α-SMA immunostaining. Cdx2 immunostaining showed that Cdx2 was not expressed in the biliary epithelium (arrow in K) and the ectopic pancreas (arrow in L) but was expressed in the duodenal epithelium (K and L). Papilla, MDP; VP, ventral pancreas. Scale bars: 50 μm.