CaM kinase II selectively signals to histone deacetylase 4 during cardiomyocyte hypertrophy
J. Clin. Invest. Johannes Backs, et al. 116:1853 doi:10.1172/JCI27438 [Go to this article.]

Figure 2
Regulation of CaMKII subcellular localization. (A) COS cells were transfected with CaMKIIδB-WT, CaMKIIδB-T287D, and CaMKIIδB-T287D/S332A. In contrast to the WT kinase, CaMKIIδB-T287D localized predominantly to the cytosol. Substitution of S332 rendered CaMKIIδB-T287D constitutively active and nuclear. (B) COS cells were cotransfected with CaMKIIδB-T287D/S332A and HDAC4 or HDAC5. Note that only HDAC4 was exported in response to the double CaMKIIδB mutant and colocalized with the kinase. (C and D) COS cells were first transfected with the indicated CaMKIIδB mutants and 12 hours later with HDAC4. Twelve hours after transfection with HDAC4, cells were treated for another 4 hours either with 1 nM leptomycin B (lower panel) or the vehicle ethanol (upper panel). Note that with leptomycin B, HDAC4 only accumulates in the nucleus in the presence of CaMKIIδB-T287D/S332A, which is active and nuclear, but not in the presence of CaMKIIδB-T287D or CaMKIIδB-T287D/K328,329N, which are active and cytosolic. (A–C) Representative images. Magnification, ×40. (D) Quantitative analysis of experiment shown in C.