NF-κB regulation of endothelial cell function during LPS-induced toxemia and cancer
J. Clin. Invest. Tatiana Kisseleva, et al. 116:2955 doi:10.1172/JCI27392 [Go to this article.]

Figure 5
Enhanced tumorigenesis in Tie-Tg mice is endothelial cell intrinsic. (A) WT and transgenic mice were engrafted with either transgenic or WT (from β-actin–GFP⁺ mice) BM. Quantification of tumor mass (percent metastasis per micrometer squared of lung tissue) demonstrated that mice with transgenic ECs (i.e., Tg, n = 4; Tg→Tg, n = 3; and β-actin→Tg, n = 6) exhibited considerably higher tumor burdens (respectively, 31%, 27%, and 23% versus 3.6%, 3.9%, and 5.2%; P < 0.0001, P < 0.007, and P < 0.001) than mice with WT ECs (i.e., WT, n = 3; WT→WT, n = 3; and Tg→WT, n = 6). Five fields were evaluated per mouse. Data represent mice from transgenic lines 2 and 4. (B) A significant increase in tumor vascularization in mice with transgenic ECs was revealed by PECAM-1 (CD31), CD34, and ICAM-1 immunostaining visualized under a ×20 objective. (C) Neovascularization in B16-BL6 cell–impregnated Matrigel was significantly increased when transplanted into Tg mice. Blood vessels were revealed by staining for PECAM-1 and visualized under ×4 and ×20 objectives, as indicated.