NF-κB regulation of endothelial cell function during LPS-induced toxemia and cancer
J. Clin. Invest. Tatiana Kisseleva, et al. 116:2955 doi:10.1172/JCI27392 [Go to this article.]

Figure 3
Transgenic ECs exhibit enhanced apoptosis. (A) Frozen hepatic tissue sections prepared from WT and Tie-Tg (line 2) mice 18 hours after PBS or LPS (2 μg/g weight) injection, stained for PECAM-1 and caspase-3 (anti–active caspase-3–specific antibody) and then visualized under a ×20 objective. Similar results were obtained with Tie–Tg line 4. (B) Immunostaining revealed a defect in the nuclear translocation of p65 in TNF-α–stimulated (100 ng/ml, 1 hour) cultured primary, pulmonary transgenic ECs. Phase-contrast bright-field (BF) images (×100 objective) of the same cells are shown in the upper panels. ECs were prepared from WT and Tie-Tg lines 2 and 4. (C) Cultured primary transgenic ECs exhibit enhanced nuclear fragmentation after TNF-α (100 ng/ml, 4.5 hours) stimulation, as revealed by DAPI staining (lower panels). Phase-contrast bright-field images (×100 objective) of the same cells are shown in the upper panels. (D) Quantification of the nuclear fragmentation/apoptosis data presented in C. Analysis represents the percentage of cells exhibiting clear nuclear fragmentation in 5 independent fields of 100 cells. Evaluation was performed by a researcher blinded to the experimental protocol.