Local InsP3-dependent perinuclear Ca2+ signaling in cardiac myocyte excitation-transcription coupling
J. Clin. Invest. Xu Wu, et al. 116:675 doi:10.1172/JCI27374 [
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Figure 5Role of CaM and protein kinase in ET-1–induced HDAC5 nuclear export. (
A) Myocytes were preincubated for 30 minutes with 4 μM K-252a (
n = 8) or 20 μM W-7 (
n = 7) followed by ET-1 (100 nM) application. (
B) ET-1–induced nuclear export of HDAC5-GFP versus mutant nonphosphorylatable HDAC5 (HDAC5-S/A-GFP;
n = 4). (
C) Adv-GFP-HDAC5–infected myocytes were pretreated with 1 μM KN-93 (
n = 6) or 10 μM Gö6976 (
n = 8), separately or simultaneously for 20–30 minutes followed by ET-1 application. (
D) Summary of HDAC5 nuclear export under different conditions. Elec stim, electrical stimulation; S/T PK, serine/threonine protein kinase; Tgc, transgenic.
