GATA-6 regulates semaphorin 3C and is required in cardiac neural crest for cardiovascular morphogenesis
J. Clin. Invest. John J. Lepore, et al. 116:929 doi:10.1172/JCI27363 [Go to this article.]

Figure 3
Expression of GATA-6 in cardiac neural crest derivatives. GATA-6 expression was examined by in situ hybridization in wild-type, SM22Cre^–GATA-6^F/F (SMCre^–), and SM22Cre⁺GATA-6^F/F (SMCre⁺) embryos. (A and B) In wild-type embryos at E9.5, GATA-6 is expressed in the cardiac outflow tract (OFT), atria (At), bulbus cordis (BC), and left ventricle. (C and D) In wild-type embryos at E11.5, GATA-6 is expressed in the SMCs (C, arrows) that populate the vascular wall of the AAo and PA, in the conotruncal endocardial cushions in the regions populated by migrating neural crest cells (D, long arrows) and in the cuff of cardiac myocytes at the base of the outflow tract (D, short arrow). (E and F) In situ hybridization studies with a GATA-6 exon 4 probe demonstrate GATA-6 expression in the OFT, At, and right and left ventricles that is markedly attenuated in E11.5 SMCre⁺ embryos (F) as compared with SMCre^– embryos (E). (G) In wild-type embryos at E12.5, GATA-6 is abundantly expressed in neural crest–derived SMCs populating the aorta (Ao), PA, and DA; in cardiac myocytes in the At, right ventricle, and left ventricle; and in the endocardial cushions (EC). Original magnification, ×100 (A, B, and E–G); ×200 (C and D).