Intestinal ABCA1 directly contributes to HDL biogenesis in vivo
J. Clin. Invest. Liam R. Brunham, et al. 116:1052 doi:10.1172/JCI27352 [Go to this article.]

Figure 1
Generation of ABCA1 intestine-specific knockout mice (Abca1^–i/–i ). (A) Southern blot of genomic liver (L) and intestine (I) DNA from mice with WT (+/+) or floxed (–i/–i) alleles in the presence of Cre recombinase. DNA was digested with EcoRV and hybridized with a probe to the genomic region between exons 44 and 45 in the Abca1 gene to produce the 6-kb WT, 7.3-kb floxed, and 4.2-kb knockout bands. (B) Quantitative real-time PCR of RNA isolated from mouse intestine. Reverse-transcribed RNA was amplified with oligos specific for Abca1 and Gapdh. (C) Western blot of tissue lysates from control (+/+) and Abca1^–i/–i (–i/–i) mice with antibodies against ABCA1, and GAPDH as loading control. (D) Quantitative real-time PCR of RNA isolated from livers of Abca1^+/+and Abca1^–i/–i mice. Reverse-transcribed RNA was amplified with oligos specific for Abca1 and Actin. (E) Representative Western blot of liver lysates from Abca1^+/+and Abca1–i/–i mice.