Trans -cyclopropanation of mycolic acids on trehalose dimycolate suppresses Mycobacterium tuberculosis –induced inflammation and virulence
J. Clin. Invest. Vivek Rao, et al. 116:1660 doi:10.1172/JCI27335 [Go to this article.]

Figure 5
Trans -cyclopropane–deficient TDM is hyperinflammatory for macrophages. (A) Dose-response curve for TNF-α induction by WT TDM (squares), ΔcmaA2 mutant TDM (circles), or ΔcmaA2 mutant complemented TDM (triangles) from RAW cells measured after 24 hours of stimulation. (B) RAW 264.7 cells were stimulated with vehicle (gray areas), WT TDM (red lines), ΔcmaA2 mutant TDM (green lines), or complemented TDM (blue lines) for 3 hours, and single cell levels of TNF-α were determined by intracellular cytokine staining for TNF-α. (C) Macrophages were stimulated with a total TDM dose of 1 μg in varying ratios of WT/ΔcmaA2 TDM as indicated, and TNF-α in culture supernatants was measured by ELISA 6 hours after stimulation.