Trans -cyclopropanation of mycolic acids on trehalose dimycolate suppresses Mycobacterium tuberculosis –induced inflammation and virulence
J. Clin. Invest. Vivek Rao, et al. 116:1660 doi:10.1172/JCI27335 [Go to this article.]

Figure 3
ΔcmaA2 M. tuberculosis is hyperinflammatory during macrophage infection. RAW 264.7 macrophages (A), BALB/C murine bone marrow–derived macrophages (B), and C57BL/6J bone marrow–derived macrophages (C) were left uninfected (light gray bars) or infected with WT M. tuberculosis (white bars), ΔcmaA2 mutant M. tuberculosis (black bars), or the complemented ΔcmaA2 mutant (dark gray bars). At the indicated time points after infection, TNF-α levels in culture supernatants were determined by ELISA. Values (pg/ml) indicate mean concentration of TNF-α in triplicate infections, and levels of statistical significance by 2-tailed Student’s t test are indicated above the bars.