Trans -cyclopropanation of mycolic acids on trehalose dimycolate suppresses Mycobacterium tuberculosis –induced inflammation and virulence
J. Clin. Invest. Vivek Rao, et al. 116:1660 doi:10.1172/JCI27335 [Go to this article.]

Figure 1
Loss of cmaA2 does not affect bacterial number but alters granuloma composition. (A) WT C57BL/6J mice were infected by aerosol with WT M. tuberculosis (white bars) or the ΔcmaA2 mutant (black bars), and bacterial titers in lungs and spleen were determined at the indicated time points by serial dilution and plating of tissue homogenates. Each bar shows the mean value of bacterial loads from 3 mice per group with error bars indicating SEM. (B) Bacterial loads in lungs of animals infected intravenously with WT M. tuberculosis (white bars) or the ΔcmaA2 mutant (black bars). (C) Altered granuloma structure in tissues upon infection with ΔcmaA2. Representative liver granulomas (3 per infecting strain) from mice infected with WT, ΔcmaA2, and complemented (comp) strain. Magnification, ×400. See Results for statistical analysis of granuloma size and cellular composition. (D) H&E-stained lungs from C57BL/6J mice infected with the indicated strains and sacrificed 6 months after infection. See Results for quantitation of granulomatous inflammatory lesions.