Role of A_2B adenosine receptor signaling in adenosine-dependent pulmonary inflammation and injury
J. Clin. Invest. Chun-Xiao Sun, et al. 116:2173 doi:10.1172/JCI27303 [Go to this article.]

Figure 7
Pulmonary fibrosis inADA^–/– mice treated with CVT-6883. (A–C) Lung sections from postnatal day 38 mice were stained with an antibody against α-SMA to visualize myofibroblast (brown stain). (A) Lung from an ADA⁺ mouse treated with vehicle. (B) Lung from an ADA^–/– mouse treated with vehicle. (C) Lung from an ADA^–/– mouse treated with CVT-6883. (D–F) Lung sections were stained with Masson’s trichrome to visualize collagen deposition (blue stain). (D) Lung from an ADA⁺ mouse treated with vehicle. (E) Lung from an ADA⁺ mouse treated with CVT-6883. (F) Lung from an ADA^–/– mouse treated with vehicle. (G) Lung from an ADA^–/– mouse treated with CVT-6883. Sections are representative of 6 different mice from each treatment. Scale bars: 100 μm. (H) Whole-lung α1-procollagen transcript levels. Data are mean pg transcript/μg RNA ± SEM. (I) Soluble collagen protein levels. Data are mean μg collagen/ml BAL fluid ± SEM. *P ≤ 0.05 versus vehicle-treated ADA⁺; ^#P ≤ 0.05 versus vehicle-treated ADA^–/–. n = 4 (ADA⁺), 8 (ADA^–/–).