Yeast zymosan, a stimulus for TLR2 and dectin-1, induces regulatory antigen-presenting cells and immunological tolerance
J. Clin. Invest. Stephanie Dillon, et al. 116:916 doi:10.1172/JCI27203 [Go to this article.]

Figure 5
Zymosan-treated DCs induce impaired activation of antigen-specific T cells in vitro via a mechanism dependent on IL-10 and lack of IL-6. (A) CD11c⁺ murine splenic DCs (10,000/well) were cultured with naive CD4⁺CD62L⁺ OT-II T cells (50,000/well), OVA_323–339 peptide (10 μg/ml), and either LPS (1 μg/ml) or zymosan (10 μg/ml) for 2 or 5 days (primary stimulation). (B) T cells were restimulated with plate-bound anti-CD3 and soluble anti-CD28 for an additional 3 days and levels of proliferation determined by incorporation of tritiated thymidine (cpm). (C) Recombinant murine IL-6 (rIL-6) or neutralizing anti–IL-10 receptor (anti–IL-10R) antibodies were added into cultures to determine the role of these cytokines in T cell proliferation induced by zymosan.