Ligation of protease-activated receptor 1 enhances αv
β6
integrin–dependent TGF-β activation and promotes acute lung injury
J. Clin. Invest. R. Gisli Jenkins, et al. 116:1606 doi:10.1172/JCI27183 [
Go to this article.]
Figure 7
Itgb6
–/–
mice instilled with TFLLRN and ventilated at high tidal volume are protected from lung edema and have reduced TGF-β
activity in the lung compared with WT mice.
(
A) WT mice (white bars) and
Itgb6–/– mice (black bars) were ventilated and instilled with peptide and control, and lung edema was measured as described previously. WT,
n = 6; and
Itgb6–/–,
n = 5. (
B) Quantification of nuclear localized phospho-Smad2 immunostaining in WT mice instilled with Hank’s or PAR1-activating peptide or
Itgb6–/– mice instilled with PAR1-activating peptide (black bar). WT mice, Hank’s,
n = 3; WT mice, TFLLRN,
n = 4;
Itgb6–/– mice,
n = 3; and
Par1–/– mice, saline,
n = 3. (
C) Representative histological sections showing nuclear localized phospho-Smad2 immunostaining in the lung of WT or
Itgb6–/– mice. Original magnification, ×400. Values are presented as mean + SEM.
*P < 0.05.
