Ligation of protease-activated receptor 1 enhances α_v β₆ integrin–dependent TGF-β activation and promotes acute lung injury
J. Clin. Invest. R. Gisli Jenkins, et al. 116:1606 doi:10.1172/JCI27183 [Go to this article.]

Figure 6
PAR1-induced pulmonary edema is mediated by the α_v β₆ integrin. (A) Mice were pretreated with control nonblocking antibody (white bars) or α_vβ₆ blocking antibody (black bars) before ventilation at 24 ml/kg and intratracheal instillation with PAR1-specific peptide (TFLLRN), vehicle (HBSS), or peptide control (FTLLRN). ELW was measured as the wet-to-dry weight ratio of excised lungs. (B) Mice were pretreated with control nonblocking antibody (white bars) or α_vβ₆ blocking antibody (black bars) before ventilation at 24 ml/kg and intratracheal instillation of peptide and controls. Lung extravascular plasma equivalents were calculated based on the extravasation of ¹²⁵I-labeled albumin into the lungs. Hank’s, n = 8; TFLLRN, n = 10; and FTLLRN, n = 11. Values are presented as mean + SEM. *P < 0.01.