Ligation of protease-activated receptor 1 enhances α_v β₆ integrin–dependent TGF-β activation and promotes acute lung injury
J. Clin. Invest. R. Gisli Jenkins, et al. 116:1606 doi:10.1172/JCI27183 [Go to this article.]

Figure 3
PAR1 agonists lead to a time-dependent increase in Smad2 phosphorylation that is α_v β₆ integrin dependent, but independent of new protein synthesis. (A and B) The time course of Smad2 phosphorylation in response to 10 μM PAR1-activating peptide SFLLRN (A) and 10 nM thrombin (B), in the presence or absence of an α_vβ₆ blocking antibody, was assessed by immunoblotting. Total Smad2 levels over time and in response to the α_vβ₆ blocking antibody were assessed as control. (C) Immunoblot showing the effect of the protein synthesis inhibitor cycloheximide (CHX) on SFLLRN-induced Smad2 phosphorylation. The effectiveness of cycloheximide as a protein synthesis inhibitor was determined by immunoblotting for total Smad2, and immunoblotting for MyD88 was used as a loading control.