Ligation of protease-activated receptor 1 enhances α_v β₆ integrin–dependent TGF-β activation and promotes acute lung injury
J. Clin. Invest. R. Gisli Jenkins, et al. 116:1606 doi:10.1172/JCI27183 [Go to this article.]

Figure 1
IMLE cells express epithelial cell markers and functional PAR1. (A) β₆ and β₄ integrin subunit cell surface expression on Itgb6^–/– Immortomouse cells was analyzed before (β6^–/–) and after (β6+) infection with a retroviral vector encoding human WT β₆. (B) Epithelial cell morphology was observed by phase-contrast light microscopy (×100), and expression of the epithelial marker cytokeratin was determined by immunofluorescence using a FITC-labeled primary antibody (×600). (C) Presence of the PAR1 receptor in IMLE cells was analyzed by Western blotting of cell lysates using a specific anti-PAR1 antibody. (D) To determine whether the PAR1 in these cells was functional, cell suspensions were treated with increasing doses of the PAR1-activating peptide SFLLRN, and increasing calcium mobilization was determined as a percentage of the positive control, 10 μM calcium ionophore.