Pathogenic role for skin macrophages in a mouse model of keratinocyte-induced psoriasis-like skin inflammation
J. Clin. Invest. Athanasios Stratis, et al. 116:2094 doi:10.1172/JCI27179 [
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Figure 2
Characterization of macrophages and dendritic cells in the skin of K14-Cre-IKK2
fl/fl
mice.
Confocal images of immunostainings of skin of untreated (
B,
D,
F,
H,
J,
K–
M,
P, and
Q) or clodronate liposome–treated (
R) K14-Cre-IKK2
fl/fl mice and of control mice (
A,
C,
E,
G,
I,
N, and
O) with antibodies against CD11b (
A and
B), CD14 (
C and
D), CD206 (
E and
F), CD83 (
G and
H), CD16/32 (
I and
J), and F4/80 (
L and
M). Respective markers are stained green; nuclei are stained red.
C and
D show unspecific staining of sebaceous glands due to the use of streptavidin-coupled fluorochrome for detection of biotinylated primary antibodies. (
K) Double staining with antibodies against CD83 (green) and CD80 (red). (
L and
M) F4/80-positive epithelium-lining macrophages in a developing lesion at P4 (
L) and in a fully developed lesion at P7 (
M). (
N–
R) Staining with mPDCA-1 antibody (green). Nuclei are stained red.
N and
P show upper dermis;
O,
Q, and
R show deep dermis and subcutis. Scale bars: 40 μm (
A–
M); 80 μm (
N–
R).
