Endothelial cell–restricted disruption of FoxM1 impairs endothelial repair following LPS-induced vascular injury
J. Clin. Invest. You-Yang Zhao, et al. 116:2333 doi:10.1172/JCI27154 [Go to this article.]

Figure 3
Time course of FoxM1 expression following LPS-induced lung microvascular injury. (A) Quantitative analysis of FoxM1 mRNA levels in mouse lungs by QRT-PCR. Total RNA was extracted from lungs collected from either WT or FoxM1 CKO mice after LPS exposure (5 mg/kg BW) at indicated times. FoxM1 mRNA levels were normalized to cyclophilin. Data are expressed as mean ± SD, n = 3–4. *P < 0.05 WT versus CKO; **P < 0.05 versus basal WT. (B) FoxM1 mRNA expression in endothelial cell–enriched primary cultures isolated from WT mouse lungs. Total RNA was extracted from endothelial cell–enriched primary cultures (passage 2) treated with either PBS (basal), 1,000 U/ml of recombinant mouse TNF-α, or 10 μM of H₂O₂ for 22 hours. Data are expressed as mean ± SD, n = 3. *P < 0.05.