Endothelial cell–restricted disruption of FoxM1 impairs endothelial repair following LPS-induced vascular injury
J. Clin. Invest. You-Yang Zhao, et al. 116:2333 doi:10.1172/JCI27154 [Go to this article.]

Figure 2
Impaired lung vascular endothelial barrier reannealing following LPS-induced microvascular injury in FoxM1 CKO mice. (A) Time course of increase in lung microvessel permeability as measured by K_f,c. Basal, 0.2 ml PBS; LPS, 5 mg/kg BW. Data are expressed as mean ± SD, n = 3–5 per group. *P < 0.001 versus basal; **P < 0.001, CKO versus WT; ^#P < 0.05, CKO versus WT. (B) Representative graphs of mouse lung wet weights continuously recorded during measurement of microvessel permeability. 48h-LPS, 48 hours after LPS challenge. (C and D) Time course of loss of isogravimetric state of lungs (edema formation) obtained from mouse lungs at indicated times after LPS challenge. WT (C) and FoxM1 CKO mice (D) are represented. Points indicate mean ± 1 SD, n = 3–5 mice per group. ^#P < 0.05 WT or CKO 6 hours after LPS challenge (6h-LPS) versus basal; ^##P < 0.05 CKO 48 hours after LPS challenge (48h-LPS) versus CKO basal or WT 48 hours after LPS challenge; ^ΧP < 0.05 CKO 96 hours after LPS challenge (96h-LPS) versus CKO basal or WT 96 hours after LPS challenge. (E) Representative micrographs of H&E staining show perivascular leukocyte infiltration in FoxM1 CKO lungs 96 hours after LPS challenge (5 mg/kg). Arrows indicate leukocyte infiltration. Br, bronchia; S, small vessels (<150 μm in diameter); L, large vessels (>150 μm in diameter). Scale bar: 50 mm. (F) Quantitative analysis of infiltrating leukocytes in lungs at 96 hours after LPS challenge. Bar graphs show infiltrating leukocytes in vessels of different diameters. Percentages of vessels of different diameters exhibiting leukocyte infiltration are shown. Data are expressed as mean ± SD, n = 3–4. ^#P < 0.05 versus WT (infiltrating leukocytes per vessel); ^##P < 0.05 versus WT (% of vessels).