Immune sensing of Candida albicans requires cooperative recognition of mannans and glucans by lectin and Toll-like receptors
J. Clin. Invest. Mihai G. Netea, et al. 116:1642 doi:10.1172/JCI27114 [Go to this article.]

Figure 6
The role of β-glucan/dectin-1 interaction for cytokine stimulation by C. albicans . The role of β-glucan/dectin-1 interaction for C. albicans–induced TNF was investigated using 2 approaches with combinations of mutant C. albicans strains and receptor blockade: (A) stimulation with the och1 NGY357 strain (29) in TLR4^–/– mice and (B) stimulation with mnt1 mnt2 NGY337 strain (27) in the presence of anti-MR antibodies in human MNCs. In both situations, the signals induced by N-linked mannosyl/MR and O-linked mannosyl/TLR4 complexes were deficient. The residual cytokine production stimulated by C. albicans in these 2 experimental conditions was completely blocked by laminarin, a ligand of dectin-1. Results (mean ± SD) are pooled data from 2 separate experiments with a total of 10 mice per group (A) or 8 human volunteers (B). *P < 0.05; **P < 0.01; ***P < 0.001 versus wild-type.