Immune sensing of Candida albicans requires cooperative recognition of mannans and glucans by lectin and Toll-like receptors
J. Clin. Invest. Mihai G. Netea, et al. 116:1642 doi:10.1172/JCI27114 [Go to this article.]

Figure 5
Differential recognition of O - andN -linked mannosyl residues by TLR4 and MR. (A) Human MNCs were stimulated with the various C. albicans strains — the parent NGY152 strain; the och1 mutant (strain NGY357; ref. 29), defective in N-linked mannosylation; and the mnt1 mnt2 mutant (strain NGY337; ref. 27), defective in O-linked mannosylation — in the presence of monoclonal antibodies against TLR4 or MR or a isotype-matched control antibody. After 24 hours’ stimulation at 37°C, supernatants were collected, and TNF concentration was measured by RIA. Results are pooled triplicate data from 2 separate experiments with a total of 8 volunteers per group. (B) Murine peritoneal macrophages from TLR4^+/+ C57BL/10J and TLR4^–/– ScCr mice were stimulated with the various C. albicans strains: NGY152, the och1 mutant (NGY357; ref. 29), and the mnt1 mnt2 mutant (NGY337; ref. 27). After 24 hours’ stimulation at 37°C, supernatants were collected, and TNF levels were determined by RIA. Results (mean ± SD) are pooled data from 2 separate experiments with a total of 10 mice per group. *P < 0.05 versus stimulation in the presence of control antibodies (A) or versus TLR4^+/+ mice (B).