Immune sensing of Candida albicans requires cooperative recognition of mannans and glucans by lectin and Toll-like receptors
J. Clin. Invest. Mihai G. Netea, et al. 116:1642 doi:10.1172/JCI27114 [Go to this article.]

Figure 4
Reintegration of the defective genes restores cytokine production. (A) MNCs were stimulated with the parent NGY152 strain, the N-linked mannosyl-defective C. albicans strain (och1; strain NGY357; ref. 29), and the complemented reintegrant och1/och1/OCH1 strain (strain NGY358). (B) Stimulation was also performed with the conditional doxycycline-dependent mutant (pTET/och1; strain NGY361; ref. 29) in both the absence and the presence of doxycycline (doxy). (C) Comparison of the mnt1 mnt2 mutant (strain NGY337; ref. 27), defective in O-linked mannosyl residues, with the mnt1 mnt2 + MNT1 reintegrant strain (strain NGY335; ref. 27). After 24 hours’ stimulation at 37°C, supernatants were collected, and cytokines were determined by RIA or ELISA. Results (mean ± SD) are pooled triplicate data from 2 separate experiments with a total of 8 volunteers per group. *P < 0.05; **P < 0.01 versus wild-type.