Immune sensing of Candida albicans requires cooperative recognition of mannans and glucans by lectin and Toll-like receptors
J. Clin. Invest. Mihai G. Netea, et al. 116:1642 doi:10.1172/JCI27114 [Go to this article.]

Figure 3
The role of N- and O-linked mannosyl residues for cytokine stimulation by C. albicans . MNCs were stimulated for various time points with the various C. albicans strains: the wild-type parent NGY152 strain; the och1 mutant (strain NGY357; ref. 29), defective in N-linked mannan; the mnt1 mnt2 mutant (strain NGY337; ref. 27), defective in O-linked mannan; and the mnn4 mutant (strain CDH15; ref. 28), defective in phosphomannan. (A and C) C. albicans concentration-dependent stimulation curves for TNF (A) and IFN-γ (C) after stimulation for 24 hours. (B and D) Time-dependent stimulation curves for the 2 cytokines when MNCs were stimulated with the various C. albicans strains. Results (mean ± SD) are pooled triplicate data from 2 separate experiments with a total of 8 volunteers per group. *P < 0.05; **P < 0.01 versus wild-type.