Immune sensing of Candida albicans
requires cooperative recognition of mannans and glucans by lectin and Toll-like receptors
J. Clin. Invest. Mihai G. Netea, et al. 116:1642 doi:10.1172/JCI27114 [
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Figure 1
Cell wall morphology in the
C. albicans
strains used in this study.
(
A–
E) TEM micrographs. (
A) Wild-type strain NGY152 [CAI-4 plus CIp10 vector]. (
B)
och1 null (strain NGY357; ref.
26) or doxycycline-regulated conditional (strain NGY361; ref.
29) mutants, which are defective in the branched outer
N-linked mannosyl chains. (
C)
mnt1 mnt2 mutant (strain NGY337; ref.
27), which lacks 4 terminal
O-linked α
1,2-mannosyl residues. (
D)
pmr1 mutant (strain NGY355; ref.
26), which has gross defects in mannosylation, characterized by absence of phosphomannan and reduced
O-linked and
N-linked glycans. (
E)
mnn4 mutant (strain CDH15; ref.
28), which lacks phosphomannan. Scale bar: 100 nm. (
F and
G) Structure of the
N- (
F) and
O-linked (
G) glycans and the site of action of deleted gene products. Man, mannosyl; β-GlcNAc, β
N-acetylglucosamine.
