Direct evidence for the role of caveolin-1 and caveolae in mechanotransduction and remodeling of blood vessels
J. Clin. Invest. Jun Yu, et al. 116:1284 doi:10.1172/JCI27100 [
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Figure 4Cav-1 is necessary for flow-induced eNOS activation. (
A–
D) Flow-induced dilations in pressurized isolated carotid arteries in the absence (circles) and presence (triangles) of
l-NAME from WT (
A; filled symbols;
n = 4 and 10 with and without
l-NAME, respectively),
Cav-1 KO (
B; open symbols;
n = 4 and 8 with and without l-NAME, respectively), and
Cav-1 RC mice (
C; gray symbols;
n = 4 and 6 with and without l-NAME, respectively). (
D) Comparison of flow-induced dilation performed in the presence of
l-NAME between the 3 strains (
n = 4 per group). The responses to flow were similar between all groups of mice in the presence of L-NAME. *
P < 0.05. (
E) Basal eNOS phosphorylation on serine 1176 was reduced in
Cav-1 KO mice and rescued in
Cav-1 RC mice. Carotid arterial lysates were prepared as described in Methods, and densitometric evaluation of the normalized ratio of phosphorylated eNOS to total eNOS is shown below. (
F) The localization of eNOS in intact carotid arteries was similar in WT,
Cav-1 KO, and
Cav-1 RC mice. Arrow reflects the direction of flow through the vessel segment.
