Alteration of intra-pancreatic target-organ specificity by abrogation of Aire in NOD mice
J. Clin. Invest. Shino Niki, et al. 116:1292 doi:10.1172/JCI26971 [Go to this article.]

Figure 3
Phenotypic analyses of lymphocytes from Aire-deficient NOD mice. (A) Production of Tregs was retained in Aire-deficient NOD mice. CD3⁺ cells from the thymus and spleen were gated and analyzed for the expression of CD4 and Foxp3. Percentages of CD4⁺Foxp3⁺ cells are indicated. In order to verify the specificity of the staining, spleen cells from control mice were stained with anti-Foxp3 mAb. Foxp3⁺ cells were detected only in the B220^– cell population, not in the B220⁺ cell population. (B) Memory/Activated (CD44^high) ICOS⁺ cells were increased in the spleens from Aire-deficient NOD mice compared with the spleens from control mice. Percentages of CD44^highICOS⁺ cells are indicated. CD4⁺ cells were gated and analyzed for CD44 and ICOS expression. (C) Serum Ig levels were elevated in Aire-deficient NOD mice (filled circles) compared with Aire-sufficient NOD littermates (open circles) except for IgG3. One mark corresponds to 1 mouse analyzed.