Platelet genomics and proteomics
in human health and disease
J. Clin. Invest. Iain C. Macaulay, et al. 115:3370 doi:10.1172/JCI26885 [
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Figure 2Analyzing the platelet proteome. 2D-GE has been extensively applied to the characterization of the platelet proteome. Published studies have analyzed the platelet proteome as a whole (
36,
38,
44) or in subfractions (
16,
40,
42). Proteins are separated on the gel by charge (pI) and molecular weight (MW). The protein spots are then digested with a proteolytic enzyme, typically trypsin, and the resultant peptides are characterized by mass spectrometry to generate partial sequence information for the protein. This information can then be used to identify the protein by comparison of the sequence with databases of known protein and nucleic acid sequences.
