Transcription factor T-bet regulates inflammatory arthritis through its function in dendritic cells
J. Clin. Invest. Jingsong Wang, et al. 116:414 doi:10.1172/JCI26631 [Go to this article.]

Figure 5
T-bet controls the production of inflammatory cytokines and chemokines in DCs but does not regulate DC migration and life span in vivo. (A) Cytokine and chemokine levels in DC culture supernatants were measured with SearchLight high dynamic range imaging and analysis system. Culture supernatants were collected from T-bet^–/– or WT DCs after in vitro culture with LPS for 20 hours. Results are from 3 independent experiments with DCs pooled from 8 mice per group in each experiment. SDF-1β, stromal cell–derived factor-1β. (B) CD11c⁺ DC counts in knee joint synovium of WT and T-bet^–/– mice at days 0 and 4 after arthritis induction (top panel). On day 4 after arthritis induction, ratios of CD11c⁺ DCs in popliteal LNs and knee joint synovium were calculated as migration index in WT and T-bet^–/– mice (bottom panel). *P > 0.05; **P < 0.005; ***P < 0.03; ^#P > 0.05; ^##P > 0.05. (C) Homing ratio (WT/T-bet KO) measurement of i.v.-injected DCs in WT and T-bet KO mice. PLN, peripheral LNs; MLN, mesenteric LNs. Results are from 9 independent experiments. (D) Homing ratio (WT/T-bet KO) of footpad-injected DCs in popliteal LNs at 20 hours after injection. Results are from 6 independent experiments.