Independent degeneration of photoreceptors and retinal pigment epithelium in conditional knockout mouse models of choroideremia
J. Clin. Invest. Tanya Tolmachova, et al. 116:386 doi:10.1172/JCI26617 [Go to this article.]

Figure 2
Analysis of Rep1 protein amount and function. (A) Total protein lysates from stomach of gunmetal (gm/gm), Chm^null/WT, and Chm^WT/WT mice were subjected to subcellular fractionation, and total (T), soluble (S), and pellet (P) fractions were immunoblotted and probed with anti-Rab27a antibody 4B12, anti-Rab8 antibody, and anti-Rab11 antibody as indicated. Calnexin was used as a control for subcellular fractionation. (B) Immunoblot of total protein lysates from lung, liver, and kidney of Chm^3lox/Y, Chm^flox/Y, and Chm^WT/Y mice probed with anti-Rep antibody J905, which recognizes both Rep1 and Rep2 proteins. (C) Total protein lysates from large intestines of Chm^3lox/Y, Chm^flox/Y, and Chm^WT/Y mice were subjected to subcellular fractionation, and total (T), soluble (S), and pellet (P) fractions were immunoblotted and probed with anti-Rab27a antibody 4B12. Calnexin was used as a control for subcellular fractionation. (D and E) In vitro prenylation was carried out on the soluble fraction of total protein lysates from large intestines (D) and eyes (E). The Chm alleles are indicated above the lanes. “MCM” indicates mice carrying the MerCreMer transgene. “+TM” indicates treatment with TM. The radiogram underneath shows a shorter exposure to film.