Loss of SPARC-mediated VEGFR-1 suppression after injury reveals a novel antiangiogenic activity of VEGF-A
J. Clin. Invest. Miho Nozaki, et al. 116:422 doi:10.1172/JCI26316 [Go to this article.]

Figure 3
Preexisting CNV inhibited subsequent CNV via VEGF-A. (A and B) Representative choroidal flat mounts show that CNV lesions (insets show magnified images) were smaller in eyes (A) where preexisting laser injury (area denoted by arrow) was performed 2 days earlier compared with those in eyes (B) without prior injury. Arrowheads denote optic nerve. Scale bars: 200 μm. (C) Preexisting laser injury created 2 days before subsequent injury markedly decreased CNV in the subsequent laser spots near (∼0.75 mm) the preexisting injury and slightly decreased CNV in spots far (∼1.25 mm) from it. Preexisting laser injury created 14 days before subsequent injury in wild-type mice did not affect CNV of subsequent laser spots either near or far from preexisting injury. *P < 0.01 compared with eyes without preexisting injury; ^#P < 0.05 compared with far lesions in the day 2 group. n = 12 per data point. (D) Neutralizing anti–VEGF-A antibody reversed this inhibition in a dose-dependent manner. ^#P < 0.05 compared with no injection. n = 12 per data point. (C and D).