Regulation of glucagon secretion by glucose transporter type 2 (glut2) and astrocyte-dependent glucose sensors
J. Clin. Invest. Nell Marty, et al. 115:3545 doi:10.1172/JCI26309 [
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Figure 3Expression of GLUT2 in astrocytes of
ripglut1;glut2–/– mice restores glucagon secretion in response to physiological hypoglycemia or cellular glucoprivation. (
A) Scheme of the GLUT2 transgene. (
B) Southern blot detection of the 3.8-kb EcoRI transgene fragment in the genomic DNA of F1 mice from 3 of the 4 lines of transgenic mice. (
C) Western blot detection of GLUT2 in whole-brain membranes of
pgfapglut2;ripglut1;glut2–/– mice. GLUT2 is detected in the brains (B) of lines 3 and 4. GLUT2 expression in liver (L) of B6 mice is presented as a control. GLUT2 is not detectable by Western blot analysis in the brains of B6 or
ripglut1;glut2–/– mice, as expected from its very low abundance. (
D) Immunofluorescence detection in the brains of mouse transgenic line 4 of GLUT2 and GFAP. Overlay of both types of staining shows expression of transgenic GLUT2 in glial cells. Scale bar: 50 μm.
